Composite
HCEC->FabI

Part:BBa_K2725001

Designed by: Ben Astles   Group: iGEM18_Edinburgh_UG   (2018-10-11)


FabI expressed under high constitutive expression cassette

FabI is an enoyl acyl-carrier-protein reductase, which is involved in type 2 fatty acid synthesis. It is the target for the biocide triclosan, which suppresses growth of E. coli DH5-alpha on agar plates at concentrations above 1 μM. When over-expressed, in this case by expression under the high constitutive expression cassette K314100, this confers resistance to triclosan as there is a sufficient level of FabI for normal function. This part was characterised by showing growth of E. coli DH5-alpha on 1 μM triclosan agar plates. This can be seen in the following link: http://2018.igem.org/File:T--Edinburgh_UG--BBa_K2725001.jpeg#file


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 470
    Illegal NheI site found at 493
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None